EFEK PEMBERIAN SENYAWA KOLKISIN TERHADAP PERTUMBUHAN TANAMAN TOMAT (Lycopersicum esculentum)
Abstrak
Keragaman genetik melalui induksi mutasi dapat dimanfaatkan sebagai salah satu metode dalam program pemuliaan tanaman untuk meningkatkan variasi genetik. Mutasi pada tanaman dapat dilakukan dengan senyawa anti mitosis seperti kolkisin. Tujuan penelitian ini adalah untuk mengetahui respon fenotipe tiga varietas tomat akibatpemberian kolkisin dan mengetahui konsentrasi kolkisin yang mampu meningkatkan pertumbuhan ketiga varietas tersebut. Rancangan percobaan menggunakan Rancangan Acak Lengkap (RAL) Faktorial dengan duafaktor. Faktor pertama berupa varietas tomat yang terdiri atas 3 jenis, yaitu varietas Rewako (V1), varietas Tymoti(V2), dan varietas Tropical ruby (V3). Faktor kedua berupa konsentrasi kolkisin dengan 4 taraf, yaitu 0% (K0), 0,2% (K1). 0,3% (K2), dan 0,4% (K3). Parameter penelitian yang diamati adalah presentase perkecambahan, tinggi tanaman, jumlah daun dan diameter batang. Hasil penelitian menunjukkan bahwa konsentrasi kolkisin tidak berpengaruh nyata terhadap presentase perkecambahan, tinggi tanaman dan jumlah daun. Diameter batang pada 0,3% dan 0,2% kolkisin lebih besar dibandingkan pada perlakuan 0% dan 0,4%.
Referensi
Aida, R. & Shibata M. 2002. High Frequency of Polyploidization in Regenerated Plant of Kalanchoe blossfeldiana Cultivar ‘Tetra Vulcan’. Plant Biothecnology, 19(5): 329- 334.
Alam, M, M., Karim, M. R., Aziz, M. A., Hossain, M. M., Ahmed. B. & Mandai, A.. 2011. Induction and Evaluation of Polyploidy in Some Local Potato Varieties of Bangladesh. Journal of Biodiversity and Environmental Sciences, 1: 16 – 21.
Albert, B, Bray, D., Lewis, J., Raff, M., Roberts, K. and Watson, D. J, 1991. Molecular Biology of the Cell. 2nd ed. Longman, London.
Al-doory, Y. 1955. The induction of polyploidy insugarcane and tomato by the use of colchicine and the study of its influence on the growth anddevelopment of the root system of tomato. LSUHistorical Dissertations and Theses. 8135. Anggraito, Y. U. 2004. Identifikasi berat, diameter, dan tebal daging buah melon (Cucumis melo L.) kultivar action 434 tetraploid akibat perlakuan kolkisin. Berala. Peneitian Hayati, 10: 37-42. Avery, G. S. jr. and Johnson, E. B. 1947. Horticulture. Mc Graw-Hill Book, New York. Barnabas, B., Obert, B. and Kovacs, G. 1999. Colchicine, an efficient genome-doubling agent for maize (Zea mays L.) microspores cultured in anthero. Plant Cell Report, 18: 858-862. Beck, S. L., R. W. Dunlop dan A. Fossey. 2003. Evaluation of Induced Polyploidy in Acacia mearnsii Through Stomatal Counts and Guard Cell Measurements. South African Journal of Botany, 69(4): 563-567. Bramley, P.M. 2000. Is Lycopene Beneficial to Human Health. Phytochemistry, 54 :.233–236. Brewbaker, J. L. 1984. Genetika Pertanian. Seri Lembaga Genetika Modern. Penerbit Gede Jaya. Jakarta. Burns, R. G. 1992. Analysis of the colchicine binding site of β-tubulin. Federation of European Biochemistry Societies, 297 (3): 205-208. Canene-Adams K., Clinton, S. K., King, J. L., Lindshield, B. L., Wharton C., Jeffery, E. & Erdman, J. W. Jr. 2004. The Growth of The Dunning R-3327-H Transplantable Prostate Adenocarcinoma in Rats Fed Diets Containing Tomato, Broccoli, Lycopene, or Receiving Finasteride Treatment. FASEB J. 18: A886 (591.4). Chen L, Y. Wang, & M. Zhao. 2006. In vitro induction and characterization of tetraploid Lychnis senno Siebold et Zucc. Horticultural Science, 41(3): 759–761 Chen, W. H., C. Y. Tang, and Y. L. Kao. 2009. Ploidy Doubling by In Vitro Culture of Excised Protocorms or Protocorm-like Bodies in Phalaenopsis Species. Plant Cell, Tissue and Organ Culture, 98: 229-238. Caperta, A. D., Delgado, M., Ressurreicao, F., Meister A., Jones, R. N., Viegas, W.& Houben, A. 2006. Colchicine-induced polyploidization depends on tubulin polymerization in C-metaphase cells. Protoplasma. 227:147-153. Cola, G. P. A. et al. 2014. In Vitro Polyploidization in Solanum lycopersicum Mill. ‘Santa Cruz Kada Gigante’. Cytologia, 79(3): 351-358. Direktorat Jenderal Hortikultura. 2013. Statistik Produksi Hortikultura Tahun 2012. Jakarta: Direktorat Jenderal Hortikultura Kementrian Perta. Eigsti, O.J. and Dustin, P. 1957. Colchicine in Agriculture, Medicine, Biology and Chemistry. The Iowa State College Press. Ameslowa Escandon, A.S., J.C. Hagiwara, L.M. Alderete. 2006. A New of Bacapa monnieri Obtain by in vitro Polyploidization. Electronic Journal of Biotechnology, 9: 181-186. Gabesius, Y.P., Siregar, L. A. M. and Husni, Y. 2012. Respon pertumbuhan dan produksi beberapa varietas kedelai (Glycine max (L.) Merril) terhadap pemberian pupuk bokashi. Jurnal Online Agroekoteknologi, 1(1).
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